Hotspot

petD

LSC · 80790-82014 · 1225 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1225
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 TCTCCGAAGAGAAAGTGGAT TCCCTAGATACAGAAGTCGTGA 45.0 45.5 55.6 57.5 1294 bp 1.0 40.7
petD_p2 TCTCCGAAGAGAAAGTGGATT TCCCTAGATACAGAAGTCGTGA 42.9 45.5 56.4 57.5 1294 bp 1.0 40.0
petD_p3 TCTCCGAAGAGAAAGTGGAT CCCTAGATACAGAAGTCGTGA 45.0 47.6 55.6 56.0 1293 bp 1.0 40.0
petD_p4 TCTCCGAAGAGAAAGTGGAT TCCCTAGATACAGAAGTCGTG 45.0 47.6 55.6 56.0 1294 bp 1.0 40.0
petD_p5 TCTCCGAAGAGAAAGTGGAT TCCCTAGATACAGAAGTCGTGAT 45.0 43.5 55.6 57.9 1294 bp 1.0 40.0