Pi
0.000405
MarkerSeek score
43.20
Primer available
yes
Label
clpP
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| clpP_p1 | TCTCAAGTTGCGGATTTACGT | AGACATTGTTACGCTTCCACA | 42.9 | 42.9 | 58.0 | 57.9 | 2132-2136 bp | 1.0 | 63.0 |
| clpP_p2 | AGTTGCGGATTTACGTAGGA | AGACATTGTTACGCTTCCACA | 45.0 | 42.9 | 56.7 | 57.9 | 2127-2131 bp | 1.0 | 60.6 |
| clpP_p3 | CTCAAGTTGCGGATTTACGT | AGACATTGTTACGCTTCCACA | 45.0 | 42.9 | 56.5 | 57.9 | 2131-2135 bp | 1.0 | 59.0 |
| clpP_p4 | TCTCAAGTTGCGGATTTACGT | GACATTGTTACGCTTCCACA | 42.9 | 45.0 | 58.0 | 56.4 | 2131-2135 bp | 1.0 | 58.9 |
| clpP_p5 | AAGTTGCGGATTTACGTAGGA | AGACATTGTTACGCTTCCACA | 42.9 | 42.9 | 57.4 | 57.9 | 2128-2132 bp | 1.0 | 58.5 |