Hotspot

atpF

LSC · 12604-13924 · 1321 bp

Pi 0.000404

MarkerSeek score 30.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1321
variable_sites: 1
pi: 0.000404
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ATGCCTACAGTAGAAGTACT	AGTGATACAAAAAGAACTCTATTTGA	40.0	26.9	52.5	55.2	1395-1405 bp	1.0	40.5
atpF_p2	AATGCCTACAGTAGAAGTACT	AGTGATACAAAAAGAACTCTATTTGA	38.1	26.9	53.4	55.2	1396-1406 bp	1.0	40.5
atpF_p3	ATGCCTACAGTAGAAGTACT	TCTTTAAGTGATACAAAAAGAACTCT	40.0	26.9	52.5	55.1	1401-1411 bp	1.0	40.5
atpF_p4	AATGCCTACAGTAGAAGTACT	TCTTTAAGTGATACAAAAAGAACTCT	38.1	26.9	53.4	55.1	1402-1412 bp	1.0	40.5
atpF_p5	ATGCCTACAGTAGAAGTACT	GTGATACAAAAAGAACTCTATTTGA	40.0	28.0	52.5	53.9	1394-1404 bp	1.0	40.5