Hotspot

accD-psaI

LSC · 62086-62804 · 719 bp

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Pi 0.000185
MarkerSeek score 43.40
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
719
variable_sites
1
pi
0.000185
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCCAATCGTACCACGTAATCC CCATTGCAATTGCCGGAAAT 47.6 45.0 57.6 58.0 887-889 bp 1.0 68.7
accD-psaI_p2 CCAATCGTACCACGTAATCCT CCATTGCAATTGCCGGAAAT 47.6 45.0 57.6 58.0 886-888 bp 1.0 68.7
accD-psaI_p3 TCCAATCGTACCACGTAATCC AAGAAGCCATTGCAATTGCC 47.6 45.0 57.6 57.9 893-895 bp 1.0 68.1
accD-psaI_p4 CCAATCGTACCACGTAATCCT AAGAAGCCATTGCAATTGCC 47.6 45.0 57.6 57.9 892-894 bp 1.0 68.0
accD-psaI_p5 TCCAATCGTACCACGTAATCC GCCGGAAATACTAGGCCTAC 47.6 55.0 57.6 57.5 876-878 bp 1.0 65.3