Hotspot

trnF-GAA-ndhJ

LSC · 50769-51616 · 848 bp

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Pi 0.00112
MarkerSeek score 40.80
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
848
variable_sites
2
pi
0.00112
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 990 bp 1.0 79.4
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 954 bp 1.0 79.1
trnF-GAA-ndhJ_p3 TGCCTAAGTAATGGTCGGGA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 1006 bp 1.0 78.4
trnF-GAA-ndhJ_p4 CTGGTAGAGCAGAGGACTGA AGTTGGATAGGATGGCCCTT 55.0 50.0 57.9 58.1 978 bp 1.0 78.4
trnF-GAA-ndhJ_p5 AATCCTCGTGTCACCAGTTC AGTTGGATAGGATGGCCCTT 50.0 50.0 57.5 58.1 957 bp 1.0 75.8