Hotspot

trnE-UUC-trnT-GGU

LSC · 33191-33822 · 632 bp

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Pi 0.000211
MarkerSeek score 41.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
632
variable_sites
1
pi
0.000211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 736 bp 1.0 78.7
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.8 58.1 715 bp 1.0 78.2
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.8 58.1 712 bp 1.0 78.1
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 714 bp 1.0 77.8
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 733 bp 1.0 77.5