Hotspot

trnC-GCA-petN

LSC · 29297-30173 · 877 bp

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Pi 0.000152
MarkerSeek score 42.90
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
877
variable_sites
1
pi
0.000152
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.1 58.0 948-950 bp 1.0 79.5
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.1 57.8 942-944 bp 1.0 78.0
trnC-GCA-petN_p3 TATCATTTTCGTGGCGGCAT TTAAAGCAGCCCAAGCAAGA 45.0 45.0 58.3 58.0 1002-1004 bp 1.0 77.5
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.4 58.0 966-968 bp 1.0 77.3
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 1002-1004 bp 1.0 76.7