Hotspot

atpH-atpI

LSC · 14639-15732 · 1094 bp

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Pi 0.000818
MarkerSeek score 44.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1094
variable_sites
2
pi
0.000818
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATAGAAGCAAGCCCGACAG GCGAATCAATGGAGGGTCAT 50.0 50.0 58.0 58.0 1183-1189 bp 1.0 79.9
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT GCGAATCAATGGAGGGTCAT 45.0 50.0 58.0 58.0 1149-1155 bp 1.0 79.8
atpH-atpI_p3 AATAGAAGCAAGCCCGACAG ATTTTTGCAACCTTAGCCGC 50.0 45.0 58.0 57.9 1214-1220 bp 1.0 79.5
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT ATTTTTGCAACCTTAGCCGC 45.0 45.0 58.0 57.9 1180-1186 bp 1.0 79.4
atpH-atpI_p5 TACCTTGACCAACTCCAGGT GCGAATCAATGGAGGGTCAT 50.0 50.0 57.9 58.0 1205-1211 bp 1.0 79.2