Hotspot

atpA-atpF

LSC · 12535-12603 · 69 bp

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Pi 0.0156
MarkerSeek score 53.80
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
69
variable_sites
3
pi
0.0156
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CGTCGGCTCTAATGGTTACC TGTTTGGGGCGATGAATGAA 55.0 45.0 58.2 58.1 116-124 bp 1.0 84.1
atpA-atpF_p2 CGTCGGCTCTAATGGTTACC ATTGGAATGTTTGGGGCGAT 55.0 45.0 58.2 58.1 123-131 bp 1.0 83.6
atpA-atpF_p3 CAATGCCGTCACCTACTTGA TGTTTGGGGCGATGAATGAA 50.0 45.0 57.9 58.1 212-220 bp 1.0 82.3
atpA-atpF_p4 CAATGCCGTCACCTACTTGA ATGTTTGGGGCGATGAATGA 50.0 45.0 57.9 57.9 213-221 bp 1.0 81.8
atpA-atpF_p5 CAATGCCGTCACCTACTTGA ATTGGAATGTTTGGGGCGAT 50.0 45.0 57.9 58.1 219-227 bp 1.0 81.7