Hotspot

psbK-psbI

LSC · 7992-8385 · 394 bp

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Pi 0.0111
MarkerSeek score 55.00
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
394
variable_sites
12
pi
0.0111
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TCTTCACGTCCAGGATTACG 50.0 50.0 57.9 57.1 529-532 bp 1.0 74.2
psbK-psbI_p2 ATGCCCGTAATACCCCTTCT TCTTCACGTCCAGGATTACG 50.0 50.0 58.2 57.1 568-571 bp 1.0 73.0
psbK-psbI_p3 TGGCAAGCTGCTGTAAGTTT TCTTCACGTCCAGGATTACG 45.0 50.0 58.3 57.1 526-529 bp 1.0 72.2
psbK-psbI_p4 TTGGCAAGCTGCTGTAAGTT TCTTCACGTCCAGGATTACG 45.0 50.0 58.3 57.1 527-530 bp 1.0 72.2
psbK-psbI_p5 TTTGGCAAGCTGCTGTAAGT TCTTCACGTCCAGGATTACG 45.0 50.0 58.3 57.1 528-531 bp 1.0 72.2