Hotspot

trnK-UUU-rps16

LSC · 4316-5168 · 853 bp

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Pi 0.00114
MarkerSeek score 42.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
853
variable_sites
3
pi
0.00114
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 982-988 bp 1.0 78.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 981-987 bp 1.0 78.7
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 991-997 bp 1.0 77.8
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 986-992 bp 1.0 77.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 985-991 bp 1.0 77.1