Hotspot

rpl14-rpl16

LSC · 77493-77610 · 118 bp

Back to Tragus

Pi 0.0169
MarkerSeek score 63.30
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
118
variable_sites
3
pi
0.0169
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 GCTCCACTGTTATCCGCTAC AATGCCCATACGAAGCCAAT 55.0 45.0 58.1 57.9 205 bp 1.0 79.4
rpl14-rpl16_p2 GCTCCACTGTTATCCGCTAC ATGCCCATACGAAGCCAATT 55.0 45.0 58.1 57.9 204 bp 1.0 79.4
rpl14-rpl16_p3 AGCTCCACTGTTATCCGCTA AATGCCCATACGAAGCCAAT 50.0 45.0 58.2 57.9 206 bp 1.0 78.8
rpl14-rpl16_p4 AGCTCCACTGTTATCCGCTA ATGCCCATACGAAGCCAATT 50.0 45.0 58.2 57.9 205 bp 1.0 78.8
rpl14-rpl16_p5 GCTCCACTGTTATCCGCTAC TGCCCATACGAAGCCAATTT 55.0 45.0 58.1 58.2 203 bp 1.0 78.8