Hotspot

trnP-UGG-psaJ

LSC · 64258-64651 · 394 bp

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Pi 0.00338
MarkerSeek score 56.60
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
394
variable_sites
2
pi
0.00338
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG AAACCTGCTAAAGCCCCAAA 45.0 45.0 57.9 57.9 503-519 bp 1.0 78.6
trnP-UGG-psaJ_p2 CAAAACAAACGCGCTACCAA AAACCTGCTAAAGCCCCAAA 45.0 45.0 58.2 57.9 504-520 bp 1.0 78.2
trnP-UGG-psaJ_p3 TTGTACCCAAAACAAACGCG AAACCTGCTAAAGCCCCAAA 45.0 45.0 57.8 57.9 511-527 bp 1.0 78.0
trnP-UGG-psaJ_p4 CCGCTACTGCCCATAAAGAA AAACCTGCTAAAGCCCCAAA 50.0 45.0 57.7 57.9 577-593 bp 1.0 76.9
trnP-UGG-psaJ_p5 TACCCAAAACAAACGCGCTA AAACCTGCTAAAGCCCCAAA 45.0 45.0 58.4 57.9 508-524 bp 1.0 76.3