Hotspot

psbE-petL

LSC · 62306-63471 · 1166 bp

Back to Tragus

Pi 0.00172
MarkerSeek score 59.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1166
variable_sites
3
pi
0.00172
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCA 45.0 45.0 58.0 57.3 1324-1326 bp 1.0 74.8
psbE-petL_p2 TGACAAATAACCAACCCGCA AGCAGCCAGTAGAAAACCAA 45.0 45.0 58.0 57.3 1323-1325 bp 1.0 74.8
psbE-petL_p3 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCA 50.0 45.0 57.9 57.3 1313-1315 bp 1.0 74.5
psbE-petL_p4 CAACCCGCAATGAATAGGGA AGCAGCCAGTAGAAAACCAA 50.0 45.0 57.9 57.3 1312-1314 bp 1.0 74.5
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC AGCAGCCAGTAGAAAACCAA 50.0 45.0 57.8 57.3 1235-1237 bp 1.0 73.7