Hotspot

rbcL-psaI

LSC · 56570-57343 · 774 bp

Back to Tragus

Pi 0.000862
MarkerSeek score 51.30
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
774
variable_sites
1
pi
0.000862
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 CATTCGACTTCAAGCCGGTA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 875-876 bp 1.0 79.9
rbcL-psaI_p2 CATTCGACTTCAAGCCGGTA AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 881-882 bp 1.0 79.3
rbcL-psaI_p3 CGCAGCTTGTGAAGTATGGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.3 58.0 904-905 bp 1.0 77.8
rbcL-psaI_p4 ATTCGACTTCAAGCCGGTAG CCATTGCAATTGCCGGAAAT 50.0 45.0 57.7 58.0 874-875 bp 1.0 77.6
rbcL-psaI_p5 TGAAATTATCCGAGCAGCGT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.7 58.0 946-947 bp 1.0 77.5