Hotspot

atpB-rbcL

LSC · 54355-55141 · 787 bp

Back to Tragus

Pi 0.000851
MarkerSeek score 58.40
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
787
variable_sites
1
pi
0.000851
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TTTGATCAATACGCCCTGCA GCTTGAAATCCAACACCTGC 45.0 50.0 57.9 57.9 903-907 bp 1.0 78.9
atpB-rbcL_p2 TATCCAGCACGGGTCCAATA GCTTGAAATCCAACACCTGC 50.0 50.0 58.2 57.9 924-928 bp 1.0 78.2
atpB-rbcL_p3 CCAGCACGGGTCCAATAATT GCTTGAAATCCAACACCTGC 50.0 50.0 58.2 57.9 921-925 bp 1.0 78.0
atpB-rbcL_p4 CCCGGGGGAAAAGTGATATC GCTTGAAATCCAACACCTGC 55.0 50.0 57.7 57.9 940-944 bp 1.0 77.7
atpB-rbcL_p5 CCGGGGGAAAAGTGATATCC GCTTGAAATCCAACACCTGC 55.0 50.0 57.7 57.9 939-943 bp 1.0 77.7