Hotspot

ndhC-trnV-UAC

LSC · 50462-51390 · 929 bp

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Pi 0.00144
MarkerSeek score 59.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
929
variable_sites
2
pi
0.00144
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAATCGGGGCTA GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 1062 bp 1.0 75.8
ndhC-trnV-UAC_p2 TCCTTCACTAATCGGGGCTA GCAGAAAAGTGCATGTTGGG 50.0 50.0 57.5 58.2 1147 bp 1.0 74.7
ndhC-trnV-UAC_p3 CTGGTCCTTCACTAATCGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.4 57.9 1066 bp 1.0 74.7
ndhC-trnV-UAC_p4 TCCTTCACTAATCGGGGCTA AGAGCCCAATGCAGAAAAGT 50.0 45.0 57.5 57.7 1157 bp 1.0 74.0
ndhC-trnV-UAC_p5 TCCTTCACTAATCGGGGCTA CGGTTCGAATCCGTATAGCC 50.0 55.0 57.5 58.3 1063 bp 1.0 74.0