Hotspot

trnF-GAA-ndhJ

LSC · 48212-48776 · 565 bp

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Pi 0.00354
MarkerSeek score 47.80
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
565
variable_sites
3
pi
0.00354
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 675 bp 1.0 78.9
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATAGGCTGGCCCTTACGTAA 50.0 50.0 58.1 58.2 665 bp 1.0 78.3
trnF-GAA-ndhJ_p3 AATCCTCGTGTCACCAGTTC TGAAAGTTGGATAGGCTGGC 50.0 50.0 57.5 57.9 678 bp 1.0 75.7
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA GAAAGTTGGATAGGCTGGCC 50.0 55.0 58.1 58.6 674 bp 1.0 75.1
trnF-GAA-ndhJ_p5 AATCCTCGTGTCACCAGTTC ATAGGCTGGCCCTTACGTAA 50.0 50.0 57.5 58.2 668 bp 1.0 75.1