Hotspot

psaA-ycf3

LSC · 42224-42859 · 636 bp

Back to Tragus

Pi 0
MarkerSeek score 59.20
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
636
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GGGGCCCTTAGCTATTGTTC TATAGCGCTTACTCCGGGAA 55.0 50.0 58.0 58.0 830-832 bp 1.0 80.0
psaA-ycf3_p2 GGGGCCCTTAGCTATTGTTC GCGCTTACTCCGGGAAATTA 55.0 50.0 58.0 58.1 826-828 bp 1.0 79.7
psaA-ycf3_p3 GTCTGGCCCATTCCTCAAAA TATAGCGCTTACTCCGGGAA 50.0 50.0 58.1 58.0 795-797 bp 1.0 79.6
psaA-ycf3_p4 GTCTGGCCCATTCCTCAAAA GCGCTTACTCCGGGAAATTA 50.0 50.0 58.1 58.1 791-793 bp 1.0 79.2
psaA-ycf3_p5 CTGGCCCATTCCTCAAAAGA TATAGCGCTTACTCCGGGAA 50.0 50.0 57.8 58.0 793-795 bp 1.0 78.4