Hotspot

atpI-atpH

LSC · 32204-32982 · 779 bp

Back to Tragus

Pi 0.00258
MarkerSeek score 60.90
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
779
variable_sites
3
pi
0.00258
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TAGCCGCAGCCTATATAGGT AGCCAATCCAGCAGCAATAA 50.0 45.0 57.5 57.9 868-871 bp 1.0 75.2
atpI-atpH_p2 TAGCCGCAGCCTATATAGGT GCAGTACCTTGACCAACTCC 50.0 55.0 57.5 58.2 912-915 bp 1.0 74.9
atpI-atpH_p3 TAGCCGCAGCCTATATAGGT AAGCAGCAGCAATTAGTGGA 50.0 45.0 57.5 57.8 845-848 bp 1.0 74.8
atpI-atpH_p4 ATTTTTGCAACGTTAGCCGC AGCCAATCCAGCAGCAATAA 45.0 45.0 58.6 57.9 881-884 bp 1.0 74.8
atpI-atpH_p5 TAGCCGCAGCCTATATAGGT GCCAATCCAGCAGCAATAAC 50.0 50.0 57.5 57.8 867-870 bp 1.0 74.7