Hotspot

petN-trnC-GCA

LSC · 18127-19044 · 918 bp

Back to Tragus

Pi 0.00218
MarkerSeek score 47.60
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
918
variable_sites
3
pi
0.00218
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGTCCACTTCTCCCCCATAC AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 1026 bp 1.0 70.9
petN-trnC-GCA_p2 GTCCACTTCTCCCCCATACT AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 1025 bp 1.0 70.9
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 967 bp 1.0 70.6
petN-trnC-GCA_p4 AGTCCACTTCTCCCCCATAC CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 1028 bp 1.0 70.4
petN-trnC-GCA_p5 GTCCACTTCTCCCCCATACT CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 1027 bp 1.0 70.4