Hotspot

rps16-trnQ-UUG

LSC · 5575-6837 · 1263 bp

Back to Tragus

Pi 0.00264
MarkerSeek score 60.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1263
variable_sites
5
pi
0.00264
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1336-1345 bp 1.0 78.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA CGCATAAAAGAAATGGGGCG 45.0 50.0 58.1 58.2 1397-1406 bp 1.0 77.9
rps16-trnQ-UUG_p3 TCATCTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1347-1356 bp 1.0 76.7
rps16-trnQ-UUG_p4 TCATCTCCTTCAAGTCGCAC CGCATAAAAGAAATGGGGCG 50.0 50.0 57.6 58.2 1408-1417 bp 1.0 76.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1340-1349 bp 1.0 73.8