Hotspot

psbA-trnK-UUU

LSC · 1150-1395 · 246 bp

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Pi 0.00542
MarkerSeek score 33.60
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
246
variable_sites
2
pi
0.00542
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 AACACCAAACCATCCGATGT ACTCCATCCGACTAGTTCCG 45.0 55.0 57.7 58.6 393 bp 1.0 73.2
psbA-trnK-UUU_p2 GCTTGTACTTTCGCGTCTCT ACTCCATCCGACTAGTTCCG 50.0 55.0 58.3 58.6 324 bp 1.0 73.2
psbA-trnK-UUU_p3 CCCCACAGGCTTGTACTTTC ACTCCATCCGACTAGTTCCG 55.0 55.0 58.5 58.6 332 bp 1.0 71.9
psbA-trnK-UUU_p4 AACACCAAACCATCCGATGT ACTAGTTCCGGGTTCGAGTC 45.0 55.0 57.7 58.8 383 bp 1.0 71.5
psbA-trnK-UUU_p5 AACACCAAACCATCCGATGT GACTAGTTCCGGGTTCGAGT 45.0 55.0 57.7 58.8 384 bp 1.0 71.5