Hotspot

rpoC2

LSC · 18974-23119 · 4146 bp

Back to Tragopogon

Pi 0.000482

MarkerSeek score 54.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4146
variable_sites: 2
pi: 0.000482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AAAAGATCGAAACGGCCTGT	CGTTGAATGGTCCTATTCGAGA	45.0	45.5	58.1	58.0	330 bp	1.0	63.3
rpoC2_p2	AAAAGATCGAAACGGCCTGT	TCGTTGAATGGTCCTATTCGAG	45.0	45.5	58.1	58.0	331 bp	1.0	63.3
rpoC2_p3	AAAAGATCGAAACGGCCTGT	TCGTTGAATGGTCCTATTCGA	45.0	42.9	58.1	56.8	331 bp	1.0	62.4
rpoC2_p4	AAAAGATCGAAACGGCCTGT	CGTTGAATGGTCCTATTCGAG	45.0	47.6	58.1	56.5	330 bp	1.0	59.8
rpoC2_p5	ACAAAAAGATCGAAACGGCC	CGTTGAATGGTCCTATTCGAGA	45.0	45.5	57.3	58.0	333 bp	1.0	58.6