Hotspot

rbcL-accD

LSC · 56600-57129 · 530 bp

Back to Tragopogon

Pi 0

MarkerSeek score 57.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 530
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCCAAATGGAGTCCTGAAC	AGCCTACATCTGTGTTCGAAC	50.0	47.6	58.1	58.0	726-728 bp	1.0	71.2
rbcL-accD_p2	TGCCAAATGGAGTCCTGAAC	GCCTACATCTGTGTTCGAACT	50.0	47.6	58.1	58.0	725-727 bp	1.0	71.2
rbcL-accD_p3	TGCGTGTGAAGTATGGAAGG	AGCCTACATCTGTGTTCGAAC	50.0	47.6	57.9	58.0	699-701 bp	1.0	71.2
rbcL-accD_p4	TGCGTGTGAAGTATGGAAGG	GCCTACATCTGTGTTCGAACT	50.0	47.6	57.9	58.0	698-700 bp	1.0	71.2
rbcL-accD_p5	CTGCCAAATGGAGTCCTGAA	GCCTACATCTGTGTTCGAACT	50.0	47.6	57.8	58.0	726-728 bp	1.0	70.3