Hotspot

matK-trnK-UUU

LSC · 3648-4377 · 730 bp

Back to Tragopogon

Pi 0.00137

MarkerSeek score 59.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 1
pi: 0.00137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	40.9	50.0	57.5	58.2	862 bp	1.0	58.8
matK-trnK-UUU_p2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	40.9	50.0	57.5	58.2	861 bp	1.0	58.8
matK-trnK-UUU_p3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	40.9	50.0	57.5	58.4	877 bp	1.0	57.0
matK-trnK-UUU_p4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	40.9	50.0	57.5	57.5	875 bp	1.0	56.5
matK-trnK-UUU_p5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	40.0	50.0	55.0	58.2	862 bp	1.0	54.8