primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TAAAGACGGTTTTCGGTGCT	GAAAGCCGTGTGCAATGAAA	20	20	45.0	45.0	57.8	57.9	0.0	0.0	0.376342	1552	1805	446-456	1.000000	78.8
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TAAAGACGGTTTTCGGTGCT	CTAGTTCCGGGTTCGAATCC	20	20	45.0	55.0	57.8	57.8	0.0	18.0	0.464081	1552	1805	361-371	1.000000	78.5
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	GTAAAGACGGTTTTCGGTGC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.437992	1552	1805	447-457	1.000000	78.3
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	GTAAAGACGGTTTTCGGTGC	CTAGTTCCGGGTTCGAATCC	20	20	50.0	55.0	57.7	57.8	0.0	18.0	0.525731	1552	1805	362-372	1.000000	78.0
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	TTTTCGGTGCTGGTTATCCA	GAAAGCCGTGTGCAATGAAA	20	20	45.0	45.0	57.4	57.9	0.0	0.0	0.703155	1552	1805	437-447	1.000000	76.2
trnK-UUU_p1	trnK-UUU	1	CACTATACATTTTCTATACACTATACA	TCGTCCTTTAAATGCTTTGA	27	20	25.9	35.0	52.9	53.1	0.0	0.0	16.971534	1806	4414	2732-2733	1.000000	40.1
trnK-UUU_p2	trnK-UUU	2	ACACTATACATTTTCTATACACTATAC	TCGTCCTTTAAATGCTTTGA	27	20	25.9	35.0	52.9	53.1	0.0	0.0	16.975082	1806	4414	2733-2734	1.000000	40.1
trnK-UUU_p3	trnK-UUU	3	CACTATACATTTTCTATACACTATACA	TTCGTCCTTTAAATGCTTTGA	27	21	25.9	33.3	52.9	54.0	0.0	0.0	17.067926	1806	4414	2733-2734	1.000000	40.1
trnK-UUU_p4	trnK-UUU	4	ACACTATACATTTTCTATACACTATAC	TTCGTCCTTTAAATGCTTTGA	27	21	25.9	33.3	52.9	54.0	0.0	0.0	17.071475	1806	4414	2734-2735	1.000000	40.1
trnK-UUU_p5	trnK-UUU	5	CACTATACATTTTCTATACACTATACA	TCGTCCTTTAAATGCTTTGATTTCA	27	25	25.9	32.0	52.9	58.0	0.0	0.0	17.125205	1806	4414	2732-2733	1.000000	40.1
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3648	4377	862-862	1.000000	58.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3648	4377	861-861	1.000000	58.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	22	20	40.9	50.0	57.5	58.4	32.7	9.4	2.892471	3648	4377	877-877	1.000000	57.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	22	20	40.9	50.0	57.5	57.5	32.7	0.0	2.953251	3648	4377	875-875	1.000000	56.5
matK-trnK-UUU_p5	matK-trnK-UUU	5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	20	20	40.0	50.0	55.0	58.2	32.7	17.1	3.163567	3648	4377	862-862	1.000000	54.8
trnS-GGA-trnC-GCA_p1	trnS-GGA-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8622	9358	888-888	1.000000	78.2
trnS-GGA-trnC-GCA_p2	trnS-GGA-trnC-GCA	2	ACGAAAACGGAAAGAGAGGG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.247447	8622	9358	894-894	1.000000	78.1
trnS-GGA-trnC-GCA_p3	trnS-GGA-trnC-GCA	3	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8622	9358	870-870	1.000000	76.0
trnS-GGA-trnC-GCA_p4	trnS-GGA-trnC-GCA	4	ACGAAAACGGAAAGAGAGGG	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.8	58.4	0.0	14.6	0.520810	8622	9358	876-876	1.000000	75.9
trnS-GGA-trnC-GCA_p5	trnS-GGA-trnC-GCA	5	CGAAAACGGAAAGAGAGGGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.532737	8622	9358	893-893	1.000000	75.8
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.700923	10868	11523	773-773	1.000000	50.5
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	20	20	40.0	50.0	54.5	58.3	0.0	0.0	3.756773	10868	11523	767-767	1.000000	50.1
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	GGAACTAAAATGAACAGTGCAGT	TCCCCGGGATTGTAGTTCAA	23	20	39.1	50.0	57.6	58.3	0.0	26.3	3.779739	10868	11523	788-788	1.000000	49.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GGAACTAAAATGAACAGTGCAGT	TTCCCCGGGATTGTAGTTCA	23	20	39.1	50.0	57.6	58.3	0.0	26.3	3.779739	10868	11523	789-789	1.000000	49.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	20	20	40.0	50.0	54.5	58.3	0.0	26.3	3.835590	10868	11523	782-782	1.000000	49.4
rpoC2_p1	rpoC2	1	AAAAGATCGAAACGGCCTGT	CGTTGAATGGTCCTATTCGAGA	20	22	45.0	45.5	58.1	58.0	0.0	8.6	2.149531	21602	21721	330-330	1.000000	63.3
rpoC2_p2	rpoC2	2	AAAAGATCGAAACGGCCTGT	TCGTTGAATGGTCCTATTCGAG	20	22	45.0	45.5	58.1	58.0	0.0	8.6	2.149531	21602	21721	331-331	1.000000	63.3
rpoC2_p3	rpoC2	3	AAAAGATCGAAACGGCCTGT	TCGTTGAATGGTCCTATTCGA	20	21	45.0	42.9	58.1	56.8	0.0	8.6	2.263236	21602	21721	331-331	1.000000	62.4
rpoC2_p4	rpoC2	4	AAAAGATCGAAACGGCCTGT	CGTTGAATGGTCCTATTCGAG	20	21	45.0	47.6	58.1	56.5	0.0	8.6	2.579750	21602	21721	330-330	1.000000	59.8
rpoC2_p5	rpoC2	5	ACAAAAAGATCGAAACGGCC	CGTTGAATGGTCCTATTCGAGA	20	22	45.0	45.5	57.3	58.0	0.0	8.6	2.740991	21602	21721	333-333	1.000000	58.6
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TATTGCTTTCATACGGCGGG	AGCGGAAACTGCTTTGAGAA	20	20	50.0	45.0	58.4	58.0	13.2	0.0	0.444007	52258	52471	366-372	1.000000	77.7
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TATTGCTTTCATACGGCGGG	CGGGTAGAGGCTATCAATGC	20	20	50.0	55.0	58.4	57.9	13.2	0.0	0.497380	52258	52471	287-293	1.000000	77.7
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TATTGCTTTCATACGGCGGG	CAAATCTAGCTCTCCGACGG	20	20	50.0	55.0	58.4	57.9	13.2	7.8	0.538239	52258	52471	316-322	1.000000	77.2
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	20	20	50.0	55.0	58.4	58.1	13.2	0.0	0.551943	52258	52471	318-324	1.000000	77.1
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	20	20	50.0	55.0	58.4	57.9	13.2	0.0	0.558762	52258	52471	320-326	1.000000	77.0
atpB-rbcL_p1	atpB-rbcL	1	ATACGCCCCAGTTTCGTTTT	CACCAGCTTTGAATCCAACAC	20	21	45.0	47.6	58.1	58.0	0.0	0.0	1.117020	54367	55171	920-922	1.000000	71.2
atpB-rbcL_p2	atpB-rbcL	2	GCCTGGCGGAAAAGCTATAT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.1	58.0	1.4	0.0	1.119705	54367	55171	966-968	1.000000	71.2
atpB-rbcL_p3	atpB-rbcL	3	ATACGCCCCAGTTTCGTTTT	ACCAGCTTTGAATCCAACACT	20	21	45.0	42.9	58.1	58.1	0.0	0.0	1.150307	54367	55171	919-921	1.000000	71.0
atpB-rbcL_p4	atpB-rbcL	4	GCCTGGCGGAAAAGCTATAT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.1	58.1	1.4	0.0	1.152993	54367	55171	965-967	1.000000	70.9
atpB-rbcL_p5	atpB-rbcL	5	ATACGCCCCAGTTTCGTTTT	ACACCAGCTTTGAATCCAACA	20	21	45.0	42.9	58.1	58.3	0.0	0.0	1.434589	54367	55171	921-923	1.000000	68.7
rbcL-accD_p1	rbcL-accD	1	TGCCAAATGGAGTCCTGAAC	AGCCTACATCTGTGTTCGAAC	20	21	50.0	47.6	58.1	58.0	11.3	21.4	1.129481	56600	57129	726-728	1.000000	71.2
rbcL-accD_p2	rbcL-accD	2	TGCCAAATGGAGTCCTGAAC	GCCTACATCTGTGTTCGAACT	20	21	50.0	47.6	58.1	58.0	11.3	27.3	1.129481	56600	57129	725-727	1.000000	71.2
rbcL-accD_p3	rbcL-accD	3	TGCGTGTGAAGTATGGAAGG	AGCCTACATCTGTGTTCGAAC	20	21	50.0	47.6	57.9	58.0	0.0	21.4	1.132487	56600	57129	699-701	1.000000	71.2
rbcL-accD_p4	rbcL-accD	4	TGCGTGTGAAGTATGGAAGG	GCCTACATCTGTGTTCGAACT	20	21	50.0	47.6	57.9	58.0	0.0	27.3	1.132487	56600	57129	698-700	1.000000	71.2
rbcL-accD_p5	rbcL-accD	5	CTGCCAAATGGAGTCCTGAA	GCCTACATCTGTGTTCGAACT	20	21	50.0	47.6	57.8	58.0	11.3	27.3	1.244203	56600	57129	726-728	1.000000	70.3
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	63090	63848	897-898	1.000000	76.8
petA-psbJ_p2	petA-psbJ	2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	63090	63848	935-936	1.000000	75.1
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	63090	63848	934-935	1.000000	75.1
petA-psbJ_p4	petA-psbJ	4	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.754934	63090	63848	903-904	1.000000	74.0
petA-psbJ_p5	petA-psbJ	5	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.761562	63090	63848	902-903	1.000000	74.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	64640	65922	1475-1475	1.000000	72.1
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	64640	65922	1398-1398	1.000000	71.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	64640	65922	1484-1484	1.000000	71.2
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	64640	65922	1489-1489	1.000000	71.1
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	64640	65922	1483-1483	1.000000	71.0
clpP_p1	clpP	1	CGAATTAACGCGAAATCCGG	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	58.0	56.9	15.9	0.0	1.139547	69931	71945	2097-2098	1.000000	70.9
clpP_p2	clpP	2	CTCGGCAGGGGTAATTTCAA	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.262153	69931	71945	2121-2122	1.000000	69.9
clpP_p3	clpP	3	AACTCGGCAGGGGTAATTTC	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.263647	69931	71945	2123-2124	1.000000	69.9
clpP_p4	clpP	4	GAAACTCGGCAGGGGTAATT	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.263647	69931	71945	2125-2126	1.000000	69.9
clpP_p5	clpP	5	TATGAAACTCGGCAGGGGTA	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	58.1	56.9	0.0	0.0	1.270657	69931	71945	2128-2129	1.000000	69.9
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.064968	93959	94537	639-639	1.000000	79.7
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.066338	93959	94537	637-637	1.000000	79.7
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	TACCATTTCACCACCAAGGC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.1	57.9	0.0	29.4	0.139265	93959	94537	627-627	1.000000	79.1
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.2	57.9	0.0	29.4	0.253803	93959	94537	634-634	1.000000	78.2
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	57.7	57.9	5.8	29.4	0.330507	93959	94537	638-638	1.000000	77.6
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	TTTAAGGCGGCCCAATATCC	20	20	50.0	50.0	58.0	58.0	17.5	17.0	0.042708	111652	112643	1179-1179	1.000000	79.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	TTTAAGGCGGCCCAATATCC	20	20	45.0	50.0	58.0	58.0	17.5	17.0	0.053997	111652	112643	1177-1177	1.000000	79.6
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	TTTAAGGCGGCCCAATATCC	20	20	45.0	50.0	58.0	58.0	17.5	17.0	0.053997	111652	112643	1178-1178	1.000000	79.6
ndhF-rpl32_p4	ndhF-rpl32	4	CTTTTTGTTGCCGTCGGAAA	TTTAAGGCGGCCCAATATCC	20	20	45.0	50.0	57.8	58.0	17.5	17.0	0.206589	111652	112643	1185-1185	1.000000	78.4
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGTCGGAAAAAG	TTTAAGGCGGCCCAATATCC	20	20	45.0	50.0	57.8	58.0	17.5	17.0	0.206589	111652	112643	1182-1182	1.000000	78.4
ccsA_p1	ccsA	1	TTCTTAATTAATTTAGGGGATTCC	ACACTACTTGATTCACGCGG	24	20	29.2	50.0	52.5	58.3	12.9	12.5	9.788141	113931	114899	1116-1116	1.000000	40.0
ccsA_p2	ccsA	2	TTCTTAATTAATTTAGGGGATTCC	CACTACTTGATTCACGCGGT	24	20	29.2	50.0	52.5	58.3	12.9	12.5	9.788141	113931	114899	1115-1115	1.000000	40.0
ccsA_p3	ccsA	3	TTCTTAATTAATTTAGGGGATTCC	ACTACTTGATTCACGCGGTT	24	20	29.2	45.0	52.5	57.5	12.9	12.5	9.948764	113931	114899	1114-1114	1.000000	40.0
ccsA_p4	ccsA	4	TTTCTTAATTAATTTAGGGGATTCC	ACACTACTTGATTCACGCGG	25	20	28.0	50.0	53.3	58.3	12.9	12.5	9.983515	113931	114899	1117-1117	1.000000	40.0
ccsA_p5	ccsA	5	TTTCTTAATTAATTTAGGGGATTCC	CACTACTTGATTCACGCGGT	25	20	28.0	50.0	53.3	58.3	12.9	12.5	9.983515	113931	114899	1116-1116	1.000000	40.0
ndhG_p1	ndhG	1	AGGAAATGCATGTGATAGCTCA	GTTTTTCCTGACCGGGTCAA	22	20	40.9	50.0	57.8	58.3	0.0	28.4	2.477902	117807	118337	652-652	1.000000	60.3
ndhG_p2	ndhG	2	AGGAAATGCATGTGATAGCTCA	ACTTCGTTTTTCCTGACCGG	22	20	40.9	50.0	57.8	58.4	0.0	0.0	2.570839	117807	118337	657-657	1.000000	59.6
ndhG_p3	ndhG	3	AGGAAATGCATGTGATAGCTCA	TTTTTCCTGACCGGGTCAAT	22	20	40.9	45.0	57.8	57.3	0.0	28.4	2.828785	117807	118337	651-651	1.000000	57.5
ndhG_p4	ndhG	4	GGAAATGCATGTGATAGCTCA	GTTTTTCCTGACCGGGTCAA	21	20	42.9	50.0	56.4	58.3	0.0	28.4	2.937590	117807	118337	651-651	1.000000	56.6
ndhG_p5	ndhG	5	AGGAAATGCATGTGATAGCTCA	CCTGACCGGGTCAATAAAGT	22	20	40.9	50.0	57.8	57.2	0.0	28.4	2.942322	117807	118337	646-646	1.000000	56.6
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.064968	142720	143298	639-639	1.000000	79.7
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.066338	142720	143298	637-637	1.000000	79.7
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	TGCAATTGCTCAGGATACCC	TACCATTTCACCACCAAGGC	20	20	50.0	50.0	57.9	58.1	29.4	0.0	0.139265	142720	143298	627-627	1.000000	79.1
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	57.9	58.2	29.4	0.0	0.253803	142720	143298	634-634	1.000000	78.2
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	57.9	57.7	29.4	5.8	0.330507	142720	143298	638-638	1.000000	77.6