Hotspot

rps16

LSC · 5059-6195 · 1137 bp

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Pi 0.0044

MarkerSeek score 61.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1137
variable_sites: 5
pi: 0.0044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGGTACTATAATTACATTACCTTCT	ATATCCCGAATTCCCATCGC	28.0	50.0	52.8	57.6	1318 bp	1.0	40.3
rps16_p2	AGGTACTATAATTACATTACCTTCTT	ATATCCCGAATTCCCATCGC	26.9	50.0	53.5	57.6	1318 bp	1.0	40.3
rps16_p3	AGGTACTATAATTACATTACCTTCT	CGCGTTGAGCCAATGACTAT	28.0	50.0	52.8	58.7	1301 bp	1.0	40.3
rps16_p4	AGGTACTATAATTACATTACCTTCTTT	ATATCCCGAATTCCCATCGC	25.9	50.0	54.2	57.6	1318 bp	1.0	40.3
rps16_p5	AGGTACTATAATTACATTACCTTCTT	CGCGTTGAGCCAATGACTAT	26.9	50.0	53.5	58.7	1301 bp	1.0	40.3