Hotspot

ndhF

SSC · 118488-120704 · 2217 bp

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Pi 0.00135

MarkerSeek score 53.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 3
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCCCAGTTTACGAGGATTC	TCTAATTGGCGAATCTGAAA	50.0	35.0	57.3	52.6	2383 bp	1.0	40.1
ndhF_p2	AGCCCAGTTTACGAGGATTC	TTCTAATTGGCGAATCTGAA	50.0	35.0	57.3	52.6	2384 bp	1.0	40.1
ndhF_p3	GCCCAGTTTACGAGGATTCT	TCTAATTGGCGAATCTGAAA	50.0	35.0	57.3	52.6	2382 bp	1.0	40.1
ndhF_p4	GCCCAGTTTACGAGGATTCT	TTCTAATTGGCGAATCTGAA	50.0	35.0	57.3	52.6	2383 bp	1.0	40.1
ndhF_p5	GCCCAGTTTACGAGGATTCT	TTTCTAATTGGCGAATCTGA	50.0	35.0	57.3	52.6	2384 bp	1.0	40.1