Hotspot

clpP

LSC · 75739-78211 · 2473 bp

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Pi 0.00202

MarkerSeek score 57.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2473
variable_sites: 5
pi: 0.00202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGATCTTAACCTGATGATTGA	AATCAATTGCCACGTTTCCA	36.4	40.0	54.7	56.5	2632-2708 bp	1.0	40.2
clpP_p2	AGATCGATCTTAACCTGATGATTGA	AATCAATTGCCACGTTTCCA	36.0	40.0	57.7	56.5	2635-2711 bp	1.0	40.2
clpP_p3	AGATCGATCTTAACCTGATGA	AATCAATTGCCACGTTTCCA	38.1	40.0	53.6	56.5	2635-2711 bp	1.0	40.2
clpP_p4	TCGATCTTAACCTGATGATTGA	ATCAATTGCCACGTTTCCAT	36.4	40.0	54.7	56.3	2631-2707 bp	1.0	40.2
clpP_p5	TCGATCTTAACCTGATGATTGA	AAATCAATTGCCACGTTTCCA	36.4	38.1	54.7	57.3	2633-2709 bp	1.0	40.2