Hotspot

ndhF-rpl32

SSC · 120705-121226 · 522 bp

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Pi 0.00585

MarkerSeek score 62.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 522
variable_sites: 3
pi: 0.00585
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAAAGTAGAAGCCCCA	GGTGGACAGTGATTTCGCTA	50.0	50.0	58.5	57.6	723-732 bp	1.0	73.9
ndhF-rpl32_p2	GGGAAAAGTAGAAGCCCCAA	GGTGGACAGTGATTTCGCTA	50.0	50.0	57.4	57.6	722-731 bp	1.0	73.5
ndhF-rpl32_p3	TTGGGAAAAGTAGAAGCCCC	GGTGGACAGTGATTTCGCTA	50.0	50.0	57.4	57.6	724-733 bp	1.0	73.5
ndhF-rpl32_p4	AGGAAGTGGAAGAAGGGGTA	GGTGGACAGTGATTTCGCTA	50.0	50.0	57.3	57.6	684-693 bp	1.0	72.5
ndhF-rpl32_p5	CAGGAAGTGGAAGAAGGGGT	GGTGGACAGTGATTTCGCTA	55.0	50.0	58.9	57.6	685-694 bp	1.0	70.9