Hotspot

rps8-rpl14

LSC · 86883-87420 · 538 bp

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Pi 0

MarkerSeek score 61.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 538
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATGCTATTCGAACCGTTCCC	CCGATCGCTAGGGAATTGAG	50.0	55.0	58.1	57.9	663-689 bp	1.0	83.0
rps8-rpl14_p2	GTGGATGCTATTCGAACCGT	CCGATCGCTAGGGAATTGAG	50.0	55.0	58.1	57.9	667-693 bp	1.0	82.9
rps8-rpl14_p3	TGTGGATGCTATTCGAACCG	CCGATCGCTAGGGAATTGAG	50.0	55.0	58.1	57.9	668-694 bp	1.0	82.9
rps8-rpl14_p4	ATGCTATTCGAACCGTTCCC	ATTTTCGGTCCGATCGCTAG	50.0	50.0	58.1	57.9	672-698 bp	1.0	82.5
rps8-rpl14_p5	GTGGATGCTATTCGAACCGT	ATTTTCGGTCCGATCGCTAG	50.0	50.0	58.1	57.9	676-702 bp	1.0	82.4