Hotspot

rbcL-accD

LSC · 61634-62721 · 1088 bp

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Pi 0.00383

MarkerSeek score 64.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 4
pi: 0.00383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGGAAAGCGATCAAGTTCGA	TGCTGAATTGAATCACCCTT	45.0	40.0	57.6	55.2	1127-1169 bp	1.0	57.4
rbcL-accD_p2	TGAAGCAGCCAAATGGAGTC	TGCTGAATTGAATCACCCTT	50.0	40.0	58.5	55.2	1173-1215 bp	1.0	57.2
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	TGCTGAATTGAATCACCCTT	50.0	40.0	57.5	55.2	1145-1187 bp	1.0	56.7
rbcL-accD_p4	GGCTGCTGCTTGTGAAGTAT	TGCTGAATTGAATCACCCTT	50.0	40.0	58.5	55.2	1146-1188 bp	1.0	56.6
rbcL-accD_p5	CAAATGGAGTCCCGAACTGG	TGCTGAATTGAATCACCCTT	55.0	40.0	58.5	55.2	1164-1206 bp	1.0	56.5