Hotspot

rps4-trnT-UGU

LSC · 50419-51088 · 670 bp

Back to Tradescantia

Pi 0.00156

MarkerSeek score 63.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 1
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	782-785 bp	1.0	79.7
rps4-trnT-UGU_p2	CTCCCAGACGGCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	58.3	58.2	751-754 bp	1.0	79.6
rps4-trnT-UGU_p3	CTCCCAGACGGCGTATTTTT	TGTATGTATGCCCGCTTAGC	50.0	50.0	58.3	57.8	801-804 bp	1.0	79.4
rps4-trnT-UGU_p4	CTCCCAGACGGCGTATTTTT	GTATGTATGCCCGCTTAGCT	50.0	50.0	58.3	57.5	800-803 bp	1.0	77.0
rps4-trnT-UGU_p5	CTCCCAGACGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.3	57.5	790-793 bp	1.0	76.7