Hotspot

trnE-UUC-trnT-GGU

LSC · 35180-36094 · 915 bp

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Pi 0.00228

MarkerSeek score 64.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 2
pi: 0.00228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CTTCTTTTCGTGGTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.2	58.1	1027-1035 bp	1.0	81.2
trnE-UUC-trnT-GGU_p2	CTTCTTTTCGTGGTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	58.2	58.1	1024-1032 bp	1.0	81.0
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	989-997 bp	1.0	80.7
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	968-976 bp	1.0	80.6
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	986-994 bp	1.0	80.5