Hotspot

psbM-trnD-GUC

LSC · 33330-34493 · 1164 bp

Back to Tradescantia

Pi 0.00175

MarkerSeek score 61.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1164
variable_sites: 2
pi: 0.00175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGAACTAAGATAAACAGTGCAGT	TTCAATTGGTCAGAGCACCG	37.5	50.0	57.9	58.5	1262-1282 bp	1.0	44.6
psbM-trnD-GUC_p2	AGGAACTAAGATAAACAGTGCAGT	CTTATTTGACGCGCCTCACT	37.5	50.0	57.9	58.6	1292-1312 bp	1.0	43.3
psbM-trnD-GUC_p3	AGGAACTAAGATAAACAGTGCAGT	TACTTATTTGACGCGCCTCA	37.5	45.0	57.9	57.3	1294-1314 bp	1.0	43.2
psbM-trnD-GUC_p4	AGGAACTAAGATAAACAGTGCAGT	GCCTCACTGGGATTGTAGTT	37.5	50.0	57.9	57.2	1280-1300 bp	1.0	42.1
psbM-trnD-GUC_p5	GGAACTAAGATAAACAGTGCAGT	TTCAATTGGTCAGAGCACCG	39.1	50.0	56.5	58.5	1261-1281 bp	1.0	41.9