Hotspot

atpH-atpI

LSC · 15342-16667 · 1326 bp

Back to Tradescantia

Pi 0

MarkerSeek score 60.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1326
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCAGCGGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	1415-1419 bp	1.0	77.3
atpH-atpI_p2	TACCCTCTACAGCTTGTCCC	ATTTTTGCAACGTTAGCCGC	55.0	45.0	58.1	58.6	1498-1502 bp	1.0	76.3
atpH-atpI_p3	AATAACGGAAGCAGCGGAAA	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.2	58.6	1416-1420 bp	1.0	76.1
atpH-atpI_p4	ATAACGGAAGCAGCGGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	1399-1403 bp	1.0	76.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	ATTTTTGCAACGTTAGCCGC	50.0	45.0	57.8	58.6	1430-1434 bp	1.0	75.8