Hotspot

trnS-GCU-trnG-UCC

LSC · 9712-10400 · 689 bp

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Pi 0.012

MarkerSeek score 69.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 8
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAGAAGTCTCGGGTAGTTGC	ACGAATCACACTTTTACCACT	55.0	38.1	58.0	55.5	826-837 bp	1.0	54.8
trnS-GCU-trnG-UCC_p2	GAAGTCTCGGGTAGTTGCAG	ACGAATCACACTTTTACCACT	55.0	38.1	58.0	55.5	824-835 bp	1.0	54.8
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	752-763 bp	1.0	54.8
trnS-GCU-trnG-UCC_p4	TCGGGTAGTTGCAGTAAACG	ACGAATCACACTTTTACCACT	50.0	38.1	57.9	55.5	818-829 bp	1.0	54.3
trnS-GCU-trnG-UCC_p5	GAAGTCTCGGGTAGTTGCAG	GAACGAATCACACTTTTACCACT	55.0	39.1	58.0	57.3	826-837 bp	1.0	53.7