Hotspot

rps16-trnQ-UUG

LSC · 6196-8475 · 2280 bp

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Pi 0.0142

MarkerSeek score 65.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2280
variable_sites: 32
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ACACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.7	58.1	2345-2349 bp	1.0	78.6
rps16-trnQ-UUG_p2	TGGACACAAAATCCACAGCT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	2383-2387 bp	1.0	78.2
rps16-trnQ-UUG_p3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	2371-2375 bp	1.0	77.8
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	2372-2376 bp	1.0	77.8
rps16-trnQ-UUG_p5	ACACGTTGCTTTCTACCACA	TTTAAACAGTGATGGGGCGT	45.0	45.0	57.7	57.7	2405-2409 bp	1.0	76.9