Hotspot

petB

LSC · 75009-76398 · 1390 bp

Back to Trachyspermum

Pi 0.00719

MarkerSeek score 49.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1390
variable_sites: 15
pi: 0.00719
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TGTTGAGAAGGTTGCCCAAA	AATGCAGCGGAAATGATTGC	45.0	45.0	57.8	58.1	2436-2438 bp	1.0	78.9
petB_p2	TGTTGAGAAGGTTGCCCAAA	GCAGCGGAAATGATTGCATT	45.0	45.0	57.8	58.1	2433-2435 bp	1.0	78.9
petB_p3	TTGTTGAGAAGGTTGCCCAA	AATGCAGCGGAAATGATTGC	45.0	45.0	57.8	58.1	2437-2439 bp	1.0	78.9
petB_p4	TTGTTGAGAAGGTTGCCCAA	GCAGCGGAAATGATTGCATT	45.0	45.0	57.8	58.1	2434-2436 bp	1.0	78.9
petB_p5	TGTTGAGAAGGTTGCCCAAA	CGGCAGATGGATAAAGGCAT	45.0	50.0	57.8	58.1	2197-2199 bp	1.0	78.7