Hotspot

ndhF-rpl32

SSC · 112665-113672 · 1008 bp

Back to Trachyspermum

Pi 0.00911
MarkerSeek score 64.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1008
variable_sites
13
pi
0.00911
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGACGGAAAAAGAAG TAAGGCTGCCCAATATCCCT 50.0 50.0 58.0 58.2 1174-1193 bp 1.0 80.7
ndhF-rpl32_p2 TGCCGACGGAAAAAGAAGAA TAAGGCTGCCCAATATCCCT 45.0 50.0 58.0 58.2 1172-1191 bp 1.0 80.6
ndhF-rpl32_p3 TTGCCGACGGAAAAAGAAGA TAAGGCTGCCCAATATCCCT 45.0 50.0 58.0 58.2 1173-1192 bp 1.0 80.6
ndhF-rpl32_p4 GTTGCCGACGGAAAAAGAAG GACAACGCTTTTAAGGCTGC 50.0 50.0 58.0 58.3 1185-1204 bp 1.0 79.5
ndhF-rpl32_p5 AAAAGAAGAAGTCCCGCTCC TAAGGCTGCCCAATATCCCT 50.0 50.0 57.8 58.2 1162-1181 bp 1.0 79.5