Hotspot

trnP-UGG-psaJ

LSC · 66625-67015 · 391 bp

Back to Trachyspermum

Pi 0.00938

MarkerSeek score 62.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 5
pi: 0.00938
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	GGTGCCACGGAAAGATATGT	45.0	50.0	57.9	58.0	455-461 bp	1.0	80.7
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	TGTCAACGCATCTGGGAAAA	45.0	45.0	57.9	58.0	534-540 bp	1.0	80.5
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	GGTGCCACGGAAAGATATGT	45.0	50.0	58.2	58.0	456-462 bp	1.0	80.2
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	TGTCAACGCATCTGGGAAAA	45.0	45.0	58.2	58.0	535-541 bp	1.0	80.1
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	TGTCAACGCATCTGGGAAAA	45.0	45.0	57.8	58.0	542-548 bp	1.0	79.9