Hotspot

petA-psbJ

LSC · 63351-64271 · 921 bp

Back to Trachyspermum

Pi 0.0166

MarkerSeek score 66.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 23
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	GCAGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	1096-1101 bp	1.0	72.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	AGCAGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	1097-1102 bp	1.0	71.2
petA-psbJ_p3	TTGCGTGTACAAGGCCTTTT	GCAGGTATTCTTGTGATCGGT	45.0	47.6	58.6	58.1	1108-1113 bp	1.0	68.4
petA-psbJ_p4	TTGCGTGTACAAGGCCTTTT	AGCAGGTATTCTTGTGATCGG	45.0	47.6	58.6	57.8	1109-1114 bp	1.0	67.6
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	GCAGGTATTCTTGTGATCGG	50.0	50.0	57.8	56.3	1096-1101 bp	1.0	67.2