Hotspot

psaA-ycf3

LSC · 42039-42760 · 722 bp

Back to Trachyspermum

Pi 0.00188

MarkerSeek score 61.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	833-842 bp	1.0	79.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	820-829 bp	1.0	79.3
psaA-ycf3_p3	TTCTTGAGAAATGACCCGGC	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.2	58.1	856-865 bp	1.0	79.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	824-833 bp	1.0	79.1
psaA-ycf3_p5	TTCTTGAGAAATGACCCGGC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.2	57.8	843-852 bp	1.0	78.8