Hotspot

trnE-UUC-trnT-GGU

LSC · 31275-32018 · 744 bp

Back to Trachyspermum

Pi 0.00541
MarkerSeek score 62.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
744
variable_sites
6
pi
0.00541
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 843-848 bp 1.0 79.6
trnE-UUC-trnT-GGU_p2 GTGTAGTACCCTACCCCCAG GAACCGATGACTTACGCCTT 60.0 50.0 58.2 58.0 878-883 bp 1.0 79.2
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 808-813 bp 1.0 78.9
trnE-UUC-trnT-GGU_p4 GTGTAGTACCCTACCCCCAG TACCGCTGAGTTAAAAGGGC 60.0 50.0 58.2 57.9 843-848 bp 1.0 78.5
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 840-845 bp 1.0 78.5