Hotspot

petA-psbJ

LSC · 68000-68950 · 951 bp

Back to Trachydium

Pi 0.0399

MarkerSeek score 76.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 108
pi: 0.0399
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGTCAGA	CTGGATTGGGTTCATCTCTGT	40.0	47.6	52.8	57.4	984-1061 bp	1.0	52.0
petA-psbJ_p2	GAGAAGGTTCAATTGTCAGAAATGA	CTGGATTGGGTTCATCTCTGT	36.0	47.6	57.8	57.4	984-1061 bp	1.0	52.0
petA-psbJ_p3	AGAAGGTTCAATTGTCAGAAATGA	CTGGATTGGGTTCATCTCTGT	33.3	47.6	56.8	57.4	983-1060 bp	1.0	52.0
petA-psbJ_p4	GAGAAGGTTCAATTGTCAGAA	CTGGATTGGGTTCATCTCTGT	38.1	47.6	53.8	57.4	984-1061 bp	1.0	52.0
petA-psbJ_p5	GAGAAGGTTCAATTGTCAGA	TGGATTGGGTTCATCTCTGT	40.0	45.0	52.8	56.1	983-1060 bp	1.0	52.0