Hotspot

trnS-GCU-trnG-UCC

LSC · 12315-12873 · 559 bp

Back to Trachydium

Pi 0.0385

MarkerSeek score 80.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 559
variable_sites: 69
pi: 0.0385
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	618-804 bp	1.0	63.4
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	667-853 bp	1.0	62.4
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	619-805 bp	1.0	61.5
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	668-854 bp	1.0	60.5
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	604-790 bp	1.0	60.4