Hotspot

rps16-trnQ-UUG

LSC · 9596-10960 · 1365 bp

Back to Trachydium

Pi 0.0472
MarkerSeek score 74.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1365
variable_sites
179
pi
0.0472
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.95
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCTACCCGAGCTCCATCAT CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.9 57.9 1342-2332 bp 1.0 90.8
rps16-trnQ-UUG_p2 GATTCCCCGCGATACACTTT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 1587-2354 bp 0.75 75.6
rps16-trnQ-UUG_p3 GATTCCCCGCGATACACTTT CCCAGCTATCCAACGAAGAC 50.0 55.0 58.1 58.1 1725-2492 bp 0.75 75.6
rps16-trnQ-UUG_p4 GATTCCCCGCGATACACTTT TTTGCCCTGGAATTGACCAA 50.0 45.0 58.1 57.9 1128-1895 bp 0.75 75.4
rps16-trnQ-UUG_p5 TTCTACCCGAGCTCCATCAT CCCAGCTATCCAACGAAGAC 50.0 55.0 57.9 58.1 1538-2305 bp 0.75 75.4