Hotspot

trnK-UUU-rps16

LSC · 7610-8481 · 872 bp

Back to Trachydium

Pi 0.047
MarkerSeek score 77.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
872
variable_sites
123
pi
0.047
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG AAAGTGTATCGCGGGGAATC 55.0 50.0 58.0 58.1 1740-1778 bp 0.75 75.0
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG AAAGTGTATCGCGGGGAATC 55.0 50.0 58.0 58.1 1739-1777 bp 0.75 75.0
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 1193-1232 bp 0.75 74.2
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 1192-1231 bp 0.75 74.2
trnK-UUU-rps16_p5 TCCCTCGCTTCATCCAAATG AAAGTGTATCGCGGGGAATC 50.0 50.0 58.0 58.1 1688-1701 bp 0.625 69.1