primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCCTTTCAGGATCAGTCGTG	AAAGTGTATCGCGGGGAATC	20	20	55.0	50.0	58.0	58.1	0.0	6.1	0.066270	7610	8481	1740-1778	0.750000	75.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	AAAGTGTATCGCGGGGAATC	20	20	55.0	50.0	58.0	58.1	0.0	6.1	0.066270	7610	8481	1739-1777	0.750000	75.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCCTTTCAGGATCAGTCGTG	TCTATCCCAATGAGCCGTCT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.103840	7610	8481	1193-1232	0.750000	74.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	TCTATCCCAATGAGCCGTCT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.103840	7610	8481	1192-1231	0.750000	74.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCCTCGCTTCATCCAAATG	AAAGTGTATCGCGGGGAATC	20	20	50.0	50.0	58.0	58.1	0.0	6.1	0.093221	7610	8481	1688-1701	0.625000	69.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TTCTACCCGAGCTCCATCAT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	57.9	57.9	8.5	0.0	0.144199	9596	10960	1342-2332	1.000000	90.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GATTCCCCGCGATACACTTT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.1	57.9	8.2	0.0	0.106629	9596	10960	1587-2354	0.750000	75.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GATTCCCCGCGATACACTTT	CCCAGCTATCCAACGAAGAC	20	20	50.0	55.0	58.1	58.1	8.2	0.0	0.109210	9596	10960	1725-2492	0.750000	75.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GATTCCCCGCGATACACTTT	TTTGCCCTGGAATTGACCAA	20	20	50.0	45.0	58.1	57.9	8.2	0.0	0.144635	9596	10960	1128-1895	0.750000	75.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTCTACCCGAGCTCCATCAT	CCCAGCTATCCAACGAAGAC	20	20	50.0	55.0	57.9	58.1	8.5	0.0	0.146779	9596	10960	1538-2305	0.750000	75.4
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	12315	12873	618-804	1.000000	63.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	12315	12873	667-853	1.000000	62.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	12315	12873	619-805	1.000000	61.5
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.2	56.2	0.0	0.0	3.927661	12315	12873	668-854	1.000000	60.5
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.6	55.5	0.9	0.0	3.945791	12315	12873	604-790	1.000000	60.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	CGATATGGCCGAGTGGTAAG	GGCTTCGTGTCGATCAATCT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.138098	32381	33076	1299-1757	1.000000	90.9
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	GGCTTCGTGTCGATCAATCT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.154233	32381	33076	1259-1717	1.000000	90.8
trnC-GCA-petN_p3	trnC-GCA-petN	3	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.217341	32381	33076	680-792	1.000000	90.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	CGATATGGCCGAGTGGTAAG	AACATGTTGCAGAACGGTCT	20	20	55.0	45.0	57.9	58.0	0.0	11.1	0.098674	32381	33076	894-922	0.750000	76.0
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	AACATGTTGCAGAACGGTCT	20	20	50.0	45.0	58.1	58.0	0.0	11.1	0.114809	32381	33076	854-882	0.750000	76.0
petN-psbM_p1	petN-psbM	1	TGGCTGAGTCTTGAATTCCG	AGTATCCCCTTCTGACTCCG	20	20	50.0	55.0	57.9	57.9	2.3	0.0	0.173870	33167	33878	1676-2132	0.875000	83.0
petN-psbM_p2	petN-psbM	2	GGCTGAGTCTTGAATTCCGT	AGTATCCCCTTCTGACTCCG	20	20	50.0	55.0	57.9	57.9	2.3	0.0	0.175333	33167	33878	1675-2131	0.875000	83.0
petN-psbM_p3	petN-psbM	3	TGGCTGAGTCTTGAATTCCG	TATTCAATTCGCGACGACGA	20	20	50.0	45.0	57.9	57.8	2.3	9.1	0.276142	33167	33878	1527-1870	0.875000	83.0
petN-psbM_p4	petN-psbM	4	AGTCCCTTTTTGACTCTGCG	AGTATCCCCTTCTGACTCCG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.190493	33167	33878	1313-1753	0.875000	82.3
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	AGTATCCCCTTCTGACTCCG	20	20	50.0	55.0	58.2	57.9	0.0	0.0	0.226077	33167	33878	1215-1655	0.875000	82.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	35123	36062	881-1124	0.875000	84.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	57.4	57.9	0.0	0.0	0.659269	35123	36062	916-1159	0.875000	81.0
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CCGTTGCCTCCTTGAAAGAG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.8	57.9	0.0	0.0	0.943401	35123	36062	882-1125	0.875000	79.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AAACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	59.1	57.9	0.0	0.0	1.193914	35123	36062	853-1096	0.875000	77.2
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	ACCGCTGAGTTAAAAGGGC	20	19	50.0	52.6	57.8	58.1	0.0	0.0	1.227191	35123	36062	880-1123	0.875000	76.8
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	49265	50113	915-980	1.000000	82.8
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	49265	50113	936-1001	1.000000	82.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	49265	50113	886-951	1.000000	82.1
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	49265	50113	888-953	1.000000	81.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	49265	50113	916-981	1.000000	80.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	51605	52355	793-872	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	51605	52355	825-904	1.000000	89.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	57.9	57.8	0.0	15.0	0.338047	51605	52355	811-890	1.000000	89.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.8	57.8	15.5	15.0	0.445800	51605	52355	843-922	1.000000	88.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATGCGATGCTCTAACCTCTG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.527459	51605	52355	795-874	1.000000	87.8
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.5	58.0	0.0	17.4	0.476915	52957	53316	402-441	1.000000	87.4
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	52957	53316	438-477	1.000000	86.6
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.610708	52957	53316	414-453	1.000000	86.2
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.564710	52957	53316	453-492	1.000000	86.0
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.631042	52957	53316	456-495	1.000000	85.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	22	20	31.8	55.0	53.9	58.1	0.0	0.0	6.230058	55447	56206	734-1127	1.000000	52.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	23	20	30.4	55.0	54.5	58.1	0.0	0.0	6.643914	55447	56206	735-1128	1.000000	52.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	23	20	30.4	55.0	54.5	58.1	0.0	0.0	6.643914	55447	56206	734-1127	1.000000	52.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	22	20	31.8	55.0	53.9	58.7	0.0	0.0	6.805875	55447	56206	719-1112	1.000000	52.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	21	20	33.3	55.0	52.3	58.1	0.0	0.0	6.808247	55447	56206	733-1126	1.000000	52.0
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGTCAGA	CTGGATTGGGTTCATCTCTGT	20	21	40.0	47.6	52.8	57.4	5.1	0.0	6.797815	68000	68950	984-1061	1.000000	52.0
petA-psbJ_p2	petA-psbJ	2	GAGAAGGTTCAATTGTCAGAAATGA	CTGGATTGGGTTCATCTCTGT	25	21	36.0	47.6	57.8	57.4	5.1	0.0	6.848415	68000	68950	984-1061	1.000000	52.0
petA-psbJ_p3	petA-psbJ	3	AGAAGGTTCAATTGTCAGAAATGA	CTGGATTGGGTTCATCTCTGT	24	21	33.3	47.6	56.8	57.4	5.1	0.0	6.852978	68000	68950	983-1060	1.000000	52.0
petA-psbJ_p4	petA-psbJ	4	GAGAAGGTTCAATTGTCAGAA	CTGGATTGGGTTCATCTCTGT	21	21	38.1	47.6	53.8	57.4	5.1	0.0	6.880942	68000	68950	984-1061	1.000000	52.0
petA-psbJ_p5	petA-psbJ	5	GAGAAGGTTCAATTGTCAGA	TGGATTGGGTTCATCTCTGT	20	20	40.0	45.0	52.8	56.1	5.1	0.0	7.034368	68000	68950	983-1060	1.000000	52.0
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	0.0	8.4	0.175650	71492	71638	222-331	1.000000	90.4
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.252065	71492	71638	214-323	1.000000	90.0
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	0.0	8.4	0.232908	71492	71638	221-330	1.000000	89.9
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	6.2	8.4	0.202860	71492	71638	249-358	1.000000	89.4
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	6.2	8.4	0.260118	71492	71638	248-357	1.000000	89.0
ndhF-rpl32_p1	ndhF-rpl32	1	CCGTGTGAACCAAAACCCTA	TAAGGCTGCCCAGTATCCTT	20	20	50.0	50.0	58.0	58.1	0.0	16.6	0.136593	117904	119054	1672-1785	0.500000	65.3
ndhF-rpl32_p2	ndhF-rpl32	2	CCGTGTGAACCAAAACCCTA	TTAAGGCTGCCCAGTATCCT	20	20	50.0	50.0	58.0	58.1	0.0	16.6	0.136593	117904	119054	1673-1786	0.500000	65.3
ndhF-rpl32_p3	ndhF-rpl32	3	GTTGCCGACGGAAAAAGAAG	AAACTAAAACGGGGCTCCAG	20	20	50.0	50.0	58.0	58.1	20.5	2.0	0.126777	117904	119054	1591-1649	0.250000	53.8
ndhF-rpl32_p4	ndhF-rpl32	4	TGCCGACGGAAAAAGAAGAA	AAACTAAAACGGGGCTCCAG	20	20	45.0	50.0	58.0	58.1	20.5	2.0	0.138066	117904	119054	1589-1647	0.250000	53.7
ndhF-rpl32_p5	ndhF-rpl32	5	CCGTGTGAACCAAAACCCTA	AAACTAAAACGGGGCTCCAG	20	20	50.0	50.0	58.0	58.1	0.0	2.0	0.124271	117904	119054	1997-2055	0.250000	53.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TACTGGGCAGCCTTAAAAGC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.173642	119220	119893	745-1064	1.000000	90.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TACTGGGCAGCCTTAAAAGC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.175013	119220	119893	746-1065	1.000000	90.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TACTGGGCAGCCTTAAAAGC	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.2	58.1	12.4	0.0	0.254755	119220	119893	1227-1546	1.000000	90.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TACTGGGCAGCCTTAAAAGC	TGAAGTAGCAAAGCCTTGGG	20	20	50.0	50.0	58.2	58.1	12.4	0.0	0.256112	119220	119893	1228-1547	1.000000	90.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TACTGGGCAGCCTTAAAAGC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.290089	119220	119893	755-1074	1.000000	89.7
ndhG-ndhI_p1	ndhG-ndhI	1	GCAATCGCACCTATTAACGC	AACAAACAGTACGAGCTGCA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.070613	124449	124935	1325-1469	1.000000	91.4
ndhG-ndhI_p2	ndhG-ndhI	2	GCAATCGCACCTATTAACGC	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.070613	124449	124935	1324-1468	1.000000	91.4
ndhG-ndhI_p3	ndhG-ndhI	3	GCAATCGCACCTATTAACGC	TGCAAGGTACATCGGTCAAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.112023	124449	124935	1309-1453	1.000000	91.1
ndhG-ndhI_p4	ndhG-ndhI	4	GCAATCGCACCTATTAACGC	TTCTTTGGGGCGTTTACCAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.087551	124449	124935	943-1087	0.875000	86.3
ndhG-ndhI_p5	ndhG-ndhI	5	GCAATCGCACCTATTAACGC	TTTCTTTGGGGCGTTTACCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.087551	124449	124935	944-1088	0.875000	86.3